Publications

Srebf1 Controls Midbrain Dopaminergic Neurogenesis

Enrique M Toledo^†, Shanzheng Yang^†, Daniel Gyllborg, Kim E van Wijk, Indranil Sinha, Manuel Varas-Godoy, Christopher L Grigsby, Peter Lönnerberg, Saiful Islam, Knut R Steffensen, Sten Linnarsson, Ernest Arenas*

^†Co-first author, *Co-corresponding authors

Published in Cell Reports
May 5, 2020. DOI: 10.1016/j.celrep.2020.107601

WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis

Karol Kaiser, Daniel Gyllborg, Jan Procházka, Alena Salašová, Petra Kompaníková, Francisco Lamus Molina, Rocio Laguna-Goya, Tomasz Radaszkiewicz, Jakub Harnoš, Michaela Procházková, David Potěšil, Roger A. Barker, Ángel Gato Casado, Zbyněk Zdráhal, Radislav Sedláček, Ernest Arenas*, J. Carlos Villaescusa*, and Vítězslav Bryja*

*Co-corresponding authors

Published in Nature Communications
April 2, 2019. DOI: 10.1038/s41467-019-09298-4

24(S),25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo

Spyridon Theofilopoulos*, Willy Antoni Abreu de Oliveira, Shanzheng Yang, Eylan Yutuc, Ahmed Saeed, Jonas Abdel-Khalik, Abbe Ullgren, Angel Cedazo-Minguez, Ingemar Björkhem, Yuqin Wang, William J. Griffiths and Ernest Arenas*

*Co-corresponding authors

Published in Journal of Biological Chemistry
January 17, 2019. DOI: 10.1074/jbc.RA118.005639

The Matricellular Protein R-Spondin 2 Promotes Midbrain Dopaminergic Neurogenesis and Differentiation

Daniel Gyllborg, Maqsood Ahmed, Enrique M. Toledo, Spyridon Theofilopoulos, Shanzheng Yang, Charles ffrench-Constant, and Ernest Arenas

Published in Stem Cell Reports
2018 Aug 23. DOI: 10.1016/j.stemcr.2018.07.014

A Zeb2-miR-200c loop controls midbrain dopaminergic neuron neurogenesis and migration

Shanzheng Yang, Enrique M Toledo, Pedro Rosmaninho, Changgeng Peng, Per Uhlén, Diogo S Castro, and Ernest Arenas

Published in Communications Biology
2018 June 25 (1); Article number: 75. DOI: 10.1038/s42003-018-0080-0

Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP

Dawei Zhang, Shanzheng Yang, Enrique M. Toledo, Daniel Gyllborg, Carmen Saltó, J. Carlos Villaescusa, and Ernest Arenas

Published in Science Signaling
2017 Aug 22;10(493). pii: eaal4165. DOI: 10.1126/scisignal.aal4165

A proteomic analysis of LRRK2 binding partners reveals interactions with multiple signaling components of the WNT/PCP pathway

Alena Salašová, Chika Yokota, David Potěšil, Zbyněk Zdráhal, Vítězslav Bryja and Ernest Arenas

Published in Molecular Neurodegeneration
2017 Jul 11;12(1):54. DOI: 10.1186/s13024-017-0193-9

Molecular analysis of the midbrain dopaminergic niche during neurogenesis

Enrique M. Toledo, Gioele La Manno, Pia Rivetti di Val Cervo, Daniel Gyllborg, Saiful Islam, Carlos Villaescusa, Sten Linnarsson, and Ernest Arenas

Published at bioRxiv
June 26, 2017; bioRxiv.org - the preprint server for Biology. DOI: 10.1101/155846

Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model

Pia Rivetti di Val Cervo, Roman A Romanov, Giada Spigolon, Débora Masini, Elisa Martín-Montañez, Enrique M Toledo, Gioele La Manno, Michael Feyder, Christian Pifl, Yi-Han Ng, Sara Padrell Sánchez, Sten Linnarsson, Marius Wernig, Tibor Harkany, Gilberto Fisone, and Ernest Arenas

Published in Nature Biotechnology
2017 May;35(5):444-452. DOI: 10.1038/nbt.3835

News and Views article at Nature Biotechnology.

Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells

Gioele La Manno^†, Daniel Gyllborg^†, Simone Codeluppi, Kaneyasu Nishimura, Carmen Salto, Amit Zeisel, Lars E. Borm, Simon R.W. Stott, Enrique M. Toledo, J. Carlos Villaescusa, Peter Lönnerberg, Jesper Ryge, Roger A. Barker, Ernest Arenas*, and Sten Linnarsson*

^†Co-first author, *Co-corresponding authors

Published in Cell
2016 Oct 6;167(2):566-580.e19. DOI: 10.1016/j.cell.2016.09.027

Supporting data and visualizations.

A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson’s disease

J Carlos Villaescusa, Bingsi Li, Enrique M Toledo, Pia Rivetti di Val Cervo, Shanzheng Yang, Simon RW Stott, Karol Kaiser, Saiful Islam, Daniel Gyllborg, Rocio Laguna‐Goya, Michael Landreh, Peter Lönnerberg, Anna Falk, Tomas Bergman, Roger A Barker, Sten Linnarsson, Licia Selleri, and Ernest Arenas

Published in The EMBO Journal
2016 Sep 15;35(18):1963-78. DOI: 10.15252/embj.201593725

Dopamine receptor antagonists enhance proliferation and neurogenesis of midbrain Lmx1a-expressing progenitors

Eva Hedlund, Laure Belnoue, Spyridon Theofilopoulos, Carmen Salto, Chris Bye, Clare Parish, Qiaolin Deng, Banafsheh Kadkhodaei, Johan Ericson, Ernest Arenas*, Thomas Perlmann*, and András Simon*

*Co-corresponding and senior author

Published in Scientific Reports
2016 Jun 1;6:26448. DOI: 10.1038/srep26448

Cholestenoic acids regulate motor neuron survival via liver X receptors

Spyridon Theofilopoulos^†, William J. Griffiths^†, Peter J. Crick, Shanzheng Yang, Anna Meljon, Michael Ogundare, Satish Srinivas Kitambi, Andrew Lockhart, Karin Tuschl, Peter T. Clayton, Andrew A. Morris, Adelaida Martinez, M. Ashwin Reddy, Andrea Martinuzzi, Maria T. Bassi, Akira Honda, Tatsuki Mizuochi, Akihiko Kimura, Hiroshi Nittono, Giuseppe De Michele, Rosa Carbone, Chiara Criscuolo, Joyce L. Yau, Jonathan R. Seckl, Rebecca Schüle, Ludger Schöls, Andreas W. Sailer, Jens Kuhle, Matthew J. Fraidakis, Jan-Åke Gustafsson, Knut R. Steffensen, Ingemar Björkhem, Patrik Ernfors, Jan Sjövall, Ernest Arenas*, and Yuqin Wang*

^†Co-first author, *Co-corresponding authors

Published in The Journal of Clinical Investigation
2014 Nov;124(11):4829-42. DOI: 10.1172/JCI68506

Cxcl12/Cxcr4 signaling controls the migration and process orientation of A9-A10 dopaminergic neurons

Shanzheng Yang, Linda C. Edman, Juan Antonio Sánchez-Alcañiz, Nicolas Fritz, Sonia Bonilla, Jonathan Hecht, Per Uhlén, Samuel J. Pleasure, J. Carlos Villaescusa, Oscar Marín, and Ernest Arenas

Published in Development
2013 Nov;140(22):4554-64. DOI: 10.1242/dev.098145

Wnt5a cooperates with canonical Wnts to generate midbrain dopaminergic neurons in vivo and in stem cells

Emma R. Andersson, Carmen Saltó, J. Carlos Villaescusa, Lukas Cajanek, Shanzheng Yang, Lenka Bryjova, Irina I. Nagy, Seppo J. Vainio, Carmen Ramirez, Vitezslav Bryja, and Ernest Arenas

Published in PNAS
2013 Feb 12;110(7):E602-10. DOI: 10.1073/pnas.1208524110

Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesis

Spyridon Theofilopoulos, Yuqin Wang, Satish Srinivas Kitambi, Paola Sacchetti, Kyle M Sousa, Karl Bodin, Jayne Kirk, Carmen Saltó, Magnus Gustafsson, Enrique M Toledo, Kersti Karu, Jan-Åke Gustafsson, Knut R Steffensen, Patrik Ernfors, Jan Sjövall, William J Griffiths, and Ernest Arenas

Published in Nature Chemical Biology
2013 Feb;9(2):126-33. DOI: 10.1038/nchembio.1156

Midbrain Dopaminergic Neuron Development at the Single Cell Level: In vivo and in Stem Cells

Emilía Sif Ásgrímsdóttir, and Ernest Arenas

Published in Frontiers in Cell and Developmental Biology
2020 June 25. DOI: 10.3389/fcell.2020.00463

Translation of WNT developmental programs into stem cell replacement strategies for the treatment of Parkinson's disease

Enrique M. Toledo, Daniel Gyllborg, and Ernest Arenas

Published in British Journal of Pharmacology
2017 May 26 [Epub ahead of print]. DOI: 10.1111/bph.13871

How to make a midbrain dopaminergic neuron

Ernest Arenas, Mark Denham, and J. Carlos Villaescusa

Published in Development
2015 Jun 1;142(11):1918-36. DOI: 10.1242/dev.097394

Liver X receptors and cholesterol metabolism: role in ventral midbrain development and neurodegeneration

Spyridon Theofilopoulos and Ernest Arenas

Published in F1000 Prime
2015 Apr 2;7:37. DOI: 10.12703/P7-37

Wnt signaling in midbrain dopaminergic neuron development and regenerative medicine for Parkinson’s disease

Ernest Arenas

Published in Journal of Molecular Cell Biology
2014 Feb;6(1):42-53. DOI: 10.1093/jmcb/mju001

Towards stem cell replacement therapies for Parkinson's disease

Ernest Arenas

Published in Biochemical and Biophysical Research Communications
2010 May 21;396(1):152-6. DOI: 10.1016/j.bbrc.2010.04.037

Transplantable midbrain dopamine neurons: A moving target

J. Carlos Villaescusa and Ernest Arenas

Published in Experimental Neurology
2010 Apr;222(2):173-8. DOI: 10.1016/j.expneurol.2009.12.028

Emerging roles of Wnts in the adult nervous system

Nibaldo C. Inestrosa and Ernest Arenas

Published in Nature Reviews Neuroscience
2010 Feb;11(2):77-86. DOI: 10.1038/nrn2755

Transcriptomic diversity of cell types across the adult human brain

imberly Siletti^†, Rebecca Hodge#, Alejandro Mossi Albiach#, Ka Wai Lee, Song-Lin Ding, Lijuan Hu, Peter Lönnerberg, Trygve Bakken, Tamara Casper, Michael Clark, Nick Dee, Jessica Gloe, Daniel Hirschstein, Nadiya V Shapovalova, C Dirk Keene, Julie Nyhus, Herman Tung, Anna Marie Yanny, Ernest Arenas, Ed S Lein, Sten Linnarsson*

Published in Science
October 13, 2023. DOI: 10.1126/science.add7046

Comprehensive cell atlas of the first-trimester developing human brain

Emelie Braun^†, Miri Danan-Gotthold^†, Lars Borm, Ka Wai Lee, Elin Vinsland, Peter Lonnerberg, Lijuan Hu, Xiaofei Li, Xiaoling He, Zaneta Andrusivova, Joakim Lundeberg, Roger Barker, Ernest Arenas^†, Erik Sundstrom^†, Sten Linnarsson*

Published in Science
October 13, 2023. DOI: 10.1126/science.adf1226

Genetic identification of cell types underlying brain complex traits yields insights into the etiology of Parkinson's disease.

Bryois, J., Skene, N. G., Hansen, T. F., Kogelman, L., Watson, H. J., Liu, Z., Eating Disorders Working Group of the Psychiatric Genomics Consortium, International Headache Genetics Consortium, 23andMe Research Team, Brueggeman, L., Breen, G., Bulik, C. M., Arenas, E., Hjerling-Leffler, J., & Sullivan, P. F.

Published in Nature Genetics
2020 May, 52:482-493 [Epub 2020 Apr 27]. DOI: 10.1038/s41588-020-0610-9

Laminin α2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development

Ahmed M, Marziali LN, Arenas E, Feltri ML, Ffrench-Constant C.

Published in Development
2019 August 29, 146(16):dev172668. DOI: 10.1242/dev.172668

The T-type Ca²⁺ Channel Ca_v3.2 Regulates Differentiation of Neural Progenitor Cells during Cortical Development via Caspase-3

Rebellato P, Kaczynska D, Kanatani S, Rayyes IA, Zhang S, Villaescusa C, Falk A, Arenas E, Hermanson O, Louhivuori L, Uhlén P.

Published in Neuroscience
2019 Jan 21;402:78-89. DOI: 10.1016/j.neuroscience.2019.01.015

Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1-/- mouse brain and plasma

William J. Griffiths, Peter J. Crick, Anna Meljon, Spyridon Theofilopoulos, Jonas Abdel-Khalik, Eylan Yutuc, Josie E. Parker, Diane E. Kelly, Steven L. Kelly, Ernest Arenas, Yuqin Wang

Published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
2018 Nov 21;pii: S1388-1981(18)30363-9. DOI: 10.1016/j.bbalip.2018.11.006

Single-cell RNA-seq analysis reveals the platinum resistance gene COX7B and the surrogate marker CD63

Nobuyuki Tanaka, Shintaro Katayama, Aparna Reddy, Kaneyasu Nishimura, Naoya Niwa, Hiroshi Hongo, Koichiro Ogihara, Takeo Kosaka, Ryuichi Mizuno, Eiji Kikuchi, Shuji Mikami, Ayako Miyakawa, Ernest Arenas, Juha Kere, Mototsugu Oya, Per Uhlén

Published in Cancer Medicine
2018 Oct 26. DOI: 10.1002/cam4.1828

Molecular architecture of the mouse nervous system

Amit Zeisel, Hannah Hochgerner, Peter Lönnerberg, Anna Johnsson, Fatima Memic, Job van der Zwan, Martin Häring, Emelie Braun, Lars E. Borm, Gioele La Manno, Simone Codeluppi, Alessandro Furlan, Kawai Lee, Nathan Skene, Kenneth D. Harris, Jens Hjerling-Leffler, Ernest Arenas, Patrik Ernfors, Ulrika Marklund, Sten Linnarsson

Published in Cell
2018 Aug 9;174(4):999-1014.e22. DOI: 10.1016/j.cell.2018.06.021

Mousebrain.org, wiki page for visualization of data

Transcriptional synergy as an emergent property defining cell subpopulation identity enables population shift

Satoshi Okawa^†, Carmen Saltó^†, Srikanth Ravichandran, Shanzheng Yang, Enrique M. Toledo, Ernest Arenas, Antonio del Sol

^†Co-first author

Published in Nature Communications
2018 Jul 3;9(1):2595. DOI: 10.1038/s41467-018-05016-8

The tyrosine Y2502.39 in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled

Katerina Strakovaa, Pierre Matricon, Chika Yokota, Elisa Arthofer, Ondrej Bernatik, David Rodriguez, Ernest Arenas, Jens Carlsson, Vitezslav Bryja, Gunnar Schulte

Published in Cellular Signalling
2017 Jun 28. pii: S0898-6568(17)30176-6. DOI: 10.1016/j.cellsig.2017.06.018

Mapping Genes for Calcium Signaling and Their Associated Human Genetic Disorders

Matthias Hörtenhuber, Enrique M. Toledo, Erik Smedler, Ernest Arenas, Seth Malmersjö, Lauri Louhivuori, Per Uhlén

Published in Bioinformatics
2017 Apr 19. DOI: 10.1093/bioinformatics/btx225

Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage

Marketa Kaucka, Tomas Zikmund, Marketa Tesarova, Daniel Gyllborg, Andreas Hellander, Josef Jaros, Jozef Kaiser, Julian Petersen, Bara Szarowska, Phillip T Newton, Vyacheslav Dyachuk, Lei Li, Hong Qian, Anne-Sofie Johansson, Yuji Mishina, Josh Currie, Elly M Tanaka, Alek Erickson, Andrew Dudley, Hjalmar Brismar, Paul Southam, Enrico Coen, Min Chen, Lee S Weinstein, Ales Hampl, Ernest Arenas, Andrei S Chagin, Kaj Fried, and Igor Adameyko

Published in eLife
2017 Apr 17;6. pii: e25902. DOI: 10.7554/eLife.25902

Analysis of neural crest-derived clones reveals novel aspects of facial development

Marketa Kaucka, Evgeny Ivashkin, Daniel Gyllborg, Tomas Zikmund, Marketa Tesarova, Jozef Kaiser, Meng Xie, Julian Petersen, Vassilis Pachnis, Silvia K. Nicolis, Tian Yu, Paul Sharpe, Ernest Arenas, Hjalmar Brismar, Hans Blom, Hans Clevers, Ueli Suter, Andrei S. Chagin, Kaj Fried, Andreas Hellander, and Igor Adameyko

Published in Science Advances
2016 Aug 3;2(8):e1600060. DOI: 10.1126/sciadv.1600060

Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system

Sueli Marques^†, Amit Zeisel^†, Simone Codeluppi, David van Bruggen, Ana Mendanha Falcão, Lin Xiao, Huiliang Li, Martin Häring, Hannah Hochgerner, Roman A. Romanov, Daniel Gyllborg, Ana B. Muñoz-Manchado, Gioele La Manno, Peter Lönnerberg, Elisa M. Floriddia, Fatemah Rezayee, Patrik Ernfors, Ernest Arenas, Jens Hjerling-Leffler, Tibor Harkany, William D. Richardson, Sten Linnarsson*, and Gonçalo Castelo-Branco*

Published in Science
2016 Jun 10;352(6291):1326-9. DOI: 10.1126/science.aaf6463

Supporting data and visualiztion

Wnt/β-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells

Agneta Månsson-Broberg, Sergey Rodin, Ivana Bulatovic, Cristián Ibarra, Marie Löfling, Rami Genead, Eva Wärdell, Ulrika Felldin, Carl Granath, Evren Alici, Katarina Le Blanc, C.I. Edvard Smith, Alena Salašová, Magnus Westgren, Erik Sundström, Per Uhlén, Ernest Arenas, Christer Sylvén, Karl Tryggvason, Matthias Corbascio, Oscar E. Simonson, Cecilia Österholm, and Karl-Henrik Grinnemo

Published in Stem Cell Reports
2016 Apr 12;6(4):607-17. DOI: 10.1016/j.stemcr.2016.02.014

Dickkopf 3 promotes the differentiation of a rostrolateral midbrain dopaminergic neuronal subset in vivo and from pluripotent stem cells in vitro in the mouse

Yoshiyasu Fukusumi, Florian Meier, Sebastian Götz, Friederike Matheus, Martin Irmler, Ruth Beckervordersandforth, Theresa Faus-Kessler, Eleonora Minina, Benedict Rauser, Jingzhong Zhang, Ernest Arenas, Elisabet Andersson, Christof Niehrs, Johannes Beckers, Antonio Simeone, Wolfgang Wurst and Nilima Prakash

Published in The Journal of Neuroscience
2015 Sep 30;35(39):13385-401. DOI: 10.1523/JNEUROSCI.1722-15.2015

Wnts are expressed in the spinal cord of adult mice and are differentially induced after injury

Carlos González-Fernández, Carmen María Fernández-Martos, Shannon D. Shields, Ernest Arenas, and Francisco Javier Rodríguez

Published in Journal of Neurotrauma
2014 Mar 15;31(6):565-81. DOI: 10.1089/neu.2013.3067

Inhibition of mitochondrial complex III blocks neuronal differentiation and maintains embryonic stem cell pluripotency

Sandro L. Pereira, Mário Grãos, Ana Sofia Rodrigues, Sandra I. Anjo, Rui A. Carvalho, Paulo J. Oliveira, Ernest Arenas, and João Ramalho-Santos

Published in PLoS ONE
2013 Dec 2;8(12):e82095. DOI: 10.1371/journal.pone.0082095

Neural progenitors organize in small-world networks to promote cell proliferation

Seth Malmersjö, Paola Rebellato, Erik Smedler, Henrike Planert, Shigeaki Kanatani, Isabel Liste, Evanthia Nanou, Hampus Sunner, Shaimaa Abdelhady, Songbai Zhang, Michael Andäng, Abdeljabbar El Manira, Gilad Silberberg, Ernest Arenas, and Per Uhlén

Published in PNAS
2013 Apr 16;110(16):E1524-32. DOI: 10.1073/pnas.1220179110

The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury

Pau González, Carmen María Fernández-Martos, Ernest Arenas, and Francisco Javier Rodríguez

Published in Journal of Neurotrauma
2013 May 15;30(10):806-17. DOI: 10.1089/neu.2012.2613